TABLE II.
Sequence and Melting Temperature (Ta) of Primers Used in Single-Cell RT-PCR of Cone Photoreceptors To Determine the Cellular Expression of NCKX Homologues
| Name | Sense | Sequence | ||
|---|---|---|---|---|
| bassNCKX2-specific primers | ||||
| SBX1 | Forward | 5′-GCACCCAAAGATGATGAGAAC-3′ | ||
| SBX2 | Reverse | 5′-AGAGCCACAGAGGAAGGATG-3′ | ||
| SBX3 | Forward | 5′-GACGAAGGTATGGGACGTTTC-3′ | ||
| SBX3M | Forward | 5′-CTCAGTGACGAAGGTATGGG-3′ | ||
| SBX4 | Reverse | 5′-GCTTTTCTTGGTTGTGTCTGG-3′ | ||
| SBX5 | Forward | 5′-GTGACGAAGGACGTTTCAAAGAG-3′ | ||
| SBX7 | Forward | 5′-CAATGCCAATGGTGTAGCTG-3′ | ||
| SBX7M | Forward | 5′-TGCCAATGGTGTAGCTGATAAA-3′ | ||
| bassNCKX1-specific primers | ||||
| SBX13 | Forward | 5′-TGAGGAGCACCATCTTCCA-3′ | ||
| SBX14 | Reverse | 5′-CTCCTCCTCCTCTTCTTCTTCTTC-3′ | ||
The annealing temperature, Ta, was 55°C for each and every one of the primers.