TABLE II.
Properties of Positions Designated as Red in the NR1 and NR2C M3 Segment
| Position | % change | Δ leak | Designation (Fig. 4 A) | Designation (Fig. 6 B) | Discrete State Dependence |
|---|---|---|---|---|---|
| NR1 | |||||
| NR1(A+3) | >0 (I) | Yes | Black | Red | No |
| NR1(A+6) | <0 (P) | Yes | Red | Red | Yes |
| NR1(A+7) | <0 (P) | Yes | Red | Red | No but k/kAPV > 100 |
| NR1(V+10) | <0 (P) | No | Red | Black | No |
| NR2C | |||||
| NR2C(A+3) | <0 (P) | Yes | Red | Red | Yes |
| NR2C(N+4) | <0 (P) | –a | Red | – | Yes |
| NR2C(A+6) | <0 (P) | Yes | Red | Red | Yes |
| NR2C(A+7) | <0 (P) | Yes | Red | Red | Yes |
| NR2C(I+10) | >0 (I) | Yes | Black | Red | No |
Listed are mutant channels, containing cysteine substitutions in the NR1 or NR2C M3 segment, that were designated red either in Fig. 4 A or Fig. 6 B. For % change, we indicated that it was <0 (potentiation) if this occurred for at least one of the test reagents, MTSEA, MTSET, or PTrEA (see Discussion). Δ leak indicates that there was a significant change in leak current following treatment with PTrEA (Fig. 6 A). A similar quantification was made for MTSET for NR2C (Table I) but not for NR1 positions (Beck et al., 1999).
a
NR2C(N+4) was not accessible to PTrEA. It was accessible to MTSET and showed a significant change in leak current (Table I).