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. 2007 Aug;130(2):203–215. doi: 10.1085/jgp.200709809

Figure 4.

Figure 4.

Glutamine-induced membrane currents and conductance are restored after blocking the catalytic activity of the carbonic anhydrase. (A) Recording of the intracellular [H+] and the membrane current, at a holding potential Vh of −40 mV, in an oocyte expressing SNAT3 with injected CAII protein, when CO2/HCO3 − was applied and removed in the presence of 10 mM glutamine at external pH 7.9 in the absence and in the presence of 10 μM ethoxyzolamide (EZA) to block the catalytic activity of CAII. (B and C) Current–voltage relationships of the isolated glutamine-induced currents in SNAT3-expressing oocytes with injected CAII protein (B) and with injection of H2O instead of CAII (C) in the absence and presence of CO2/HCO3 −. (D) The glutamine-induced membrane slope conductance, ΔGm, in SNAT3-expressing oocytes with and without injected CAII at pH 7.9 in the absence and presence of EZA in HEPES- and in CO2/HCO3 −-buffered solution.