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. 2007 Aug;130(2):203–215. doi: 10.1085/jgp.200709809

Figure 7.

Figure 7.

Effect of different CAII mutants coexpressed on SNAT3-associated membrane conductance. (A and B) Recordings of intracellular [H+] and membrane current (Im) at a holding potential Vh of −40 mV, in SNAT3-expressing oocytes during application of 10 mM glutamine to activate SNAT3 in the presence of CO2/HCO3 − before and after blocking CAII with EZA (10 μM) at pH 7.9 with the catalytically inactive CAII mutant V143Y coexpressed (SNAT3+CAII-V143Y; A), and with the N terminus CAII mutant HEX coexpressed (SNAT3+CAII-HEX; B). Current–voltage relationships of the isolated glutamine-induced membrane currents in SNAT3-expressing oocytes with coexpressed CAII mutants V143Y or HEX in the absence (C) and in the presence of EZA (D). (E) Glutamine-induced membrane conductance changes (ΔGm) of SNAT3-expressing oocytes without CAII coexpressed (SNAT3+H2O), and with the mutant CAII-V143Y or CAII-HEX coexpressed in the absence and presence of EZA. Note the recovery of the membrane conductance in oocytes expressing SNAT3-CAII-HEX in the presence of EZA. (F) Rate of cytosolic acidification (ΔH+/t) induced by CO2/HCO3 − in oocytes without CAII (SNAT3+H2O), and with the mutants CAII-V143Y or CAII-HEX coexpressed.