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. 2007 Aug;130(2):133–144. doi: 10.1085/jgp.200609575

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — [Ca²⁺]_i oscillations originate from the SR. (A) Application of thapsigargin (2 μM) led to a complete halt of both [Ca²⁺]_i (top) and E_m (bottom) oscillations in patch-clamped cells. (B–E) Spontaneous activity ([Ca²⁺]_i oscillations or transients, left) in non (B and C) or perforated patch-clamped (E) and Fura-2-AM–loaded cardiomyocytes was unaltered in the majority of cells upon application of ryanodine (20 μM; B, middle) or 2-APB (100 μM; C, middle), whereas application of both blocked spontaneous activity in all cells (B, C, and E, right). The traces of individual cells (2 in B and 4 in C) were vertically shifted to be better visible. Cells with [Ca²⁺]_i transients could still be field stimulated (C, right, two top traces); APs could be evoked using brief (2-ms) current injections (E, right). (D) Analysis of the experiments shown in B and C.