Figure 3.
Phosducin knockout rods show normal recovery from larger bleaches. Recovery of the dark current (Id) as a function of time following substantial bleaching exposures in different WT (black, n = 7) and Pd−/− (red, n = 9) rods. The brief (1.4 s) light exposure that bleached an average of 2.6% of the rhodopsin in WT rods suppressed Id strongly, and it recovered to its dark value slowly, over a period of hundreds of seconds (see Materials and methods). Bleaches that drove transduction to a similar extent in Pd−/− rods (3.7% bleach) yielded responses that recovered along a similar time course.