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. 2007 Oct;130(4):389–398. doi: 10.1085/jgp.200709783

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Copyright © 2007, The Rockefeller University Press

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Figure 2. — Reduced glutathione levels in cells incubated for 7, 30, or 60 min at pO₂ of 20, 5, or 0.5. Data are means of five independent experiments ± SEM. * denotes P < 0.05 compared with the level at 20 kPa after 7 min of incubation. Inset represents the changes in GSH:GSSG half-reduction potential Ehc in cells exposed to pO₂ of 20, 5, or 0.5 kPa for 60 min. Ehc was calculated using the following equation (for details see Schafer and Buettner, 2001): Ehc = −240 – (59.55/2)*log([GSH]/[GSSG]) for the T 25°C, pH 7.0.