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Effects of K149 side chain identity on voltage-dependent gating. (A) Example of a voltage pulse protocol (top left) and of current responses from a selection of K149 mutants. Such current responses were used to derive open probability (P o) as a function of voltage (V). (B). Plots of open probability were fit as previously described (Engh et al., 2007) (solid lines), and the resulting gating parameters are shown in Table I.
