Figure 3.

K149 mutants have chloride dependences similar to wild type. (A) Examples of K149L currents in 5 and 310 mM external chloride. Voltage protocols used were similar to the example shown in Fig. 2 A. Such current responses were used to derive open probability (P _o) versus voltage (V) (B–E). P _o(V) plots were fit as previously described (Engh et al., 2007) (solid lines), and gating parameters are shown in Table S1. Points show mean values ± SEM for 5–21 patches for all external chloride concentrations except 610 mM, for which 3–9 patches were used.