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. 2007 Oct;130(4):379–388. doi: 10.1085/jgp.200609730

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Copyright © 2007, The Rockefeller University Press

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Figure 3. — Differential accessibility of α_1S C-terminal sites to streptavidin in nonfixed myotubes. (A) Myotubes expressing YFP-α_1S1860-BAD did not display binding of streptavidin that colocalized with loci of fluorescent DHPRs. (B) Myotubes expressing YFP-α_1S1667-BAD displayed incomplete colocalization between loci of DHPRs and streptavidin binding. The arrow indicates a cluster of DHPRs with substantial binding of streptavidin, and the arrowhead a cluster of DHPRS showing very little binding of streptavidin. (C) Nearly complete colocalization was observed for YFP-α_1S(1667-BAD)1860. Bars, 5 μm.