Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Oct;130(4):415–425. doi: 10.1085/jgp.200709771

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — Numerical computation predicts mutant behavior. (A) Schematic representation of the energy profile used for computer modeling of the three barriers–two sites channel model. Barriers (P1, P2, P3) and wells (DCS and EEEE) are spaced equally (see Materials and methods). Membrane voltage drops across the narrow portion of the channel. (B, left) Simulated ICa/IBa ratios calculated for decreasing free energies at the external DCS locus of the Ca_V2.3 channel. The bell-shaped relation obtained for the DCS locus reached a maximum when DCS ΔG° equals the energy of the internal site. (B, right) Simulated current–mole fraction curves with three out of the four decreasing DCS ΔG° depicted on the left. These curves were labeled Ca_V1.2, Ca_V2.3(DEEE), and Ca_V2.3(GGGG), after comparison with original traces shown in Figs. 1 and 3, and suspected variations in free energy at DCS locus due to mutation. (C) Simulated ICa/IBa ratios calculated for decreasing free energies at the external DCS locus of the Ca_V3.1 channel. Energy profile of Ca_V3.1 was first adapted from Ca_V2.3 by adjusting the ΔG° at the EEEE locus considering that this locus in LVA channels (EEDD) has lower affinity for Ca²⁺. Simulated current–mole fraction curves were then obtained by decreasing ΔG° at the DCS locus. Traces were labeled after comparison with original traces shown in Fig. 1 and suspected variations in free energy at DCS for the purpose of discussion.