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Effects of external chloride on gating. (A) Voltage pulse protocol (top left) and current responses in 5, 110, and 310 mM external chloride. (B) Tail-current analysis was used to determine the apparent open probability (Po) as a function of voltage (V) (see Materials and methods) (n = 5–26 for external chloride concentrations <600 mM, and n = 2–5 for 610 mM). These plots were fit as described in Materials and methods (solid lines), and gating parameters were derived (Table II).
