TABLE II.

Φ Values

Position	Φ (±SD)	Fold-change in Keq
A270	0.65 (0.07)	150
V271	–	2
P272	0.62 (0.05)	11850
L273	–	4
I274	0.62 (0.04)	2014
G275	0.65 (0.06)	88
K276	–	5
V132	0.75 (0.08)	2820
T133	–	6
H134	0.71 (0.03)	10
F135	0.75 (0.12)	18
F137	–	4

The fold-change in K_eq is for the most extreme constructs at each position. The REFERs are shown in Figs. 3 and 5. Φ values could not be determined accurately for positions where the fold-change in K_eq was <10-fold.