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. 2007 Dec;130(6):601–610. doi: 10.1085/jgp.200709828

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — State dependence of Ba²⁺ block. (A) Open WT SK2 channels were closed by removal of Ca²⁺ from the cytosolic face of an inside-out membrane patch, then 1 μM Ba²⁺ applied to the closed channels for 10 s. A solution containing 2 μM Ca²⁺ and 1 μM Ba²⁺ was then perfused onto the patch, resulting in channel opening and onset of block. Inset, expanded time course of channel opening followed by onset of Ba²⁺ block. The dashed line below the trace indicates the zero current level. (B) A single patch containing WT SK2 channels was exposed in the closed state to 1 μM Ba²⁺ according to the protocol outlined in A for 1, 3, 10, or 100 s and the four consecutive recordings overlaid. Ba²⁺ was washed from the patch between each of the four traces, and overlapping portions of the traces were removed for display. The dashed line is aligned to the peak current amplitude upon opening after the 1-s closed state Ba²⁺ exposure and does not have any analytical meaning. (C) Average peak current upon opening of channels (I_post) normalized to the current amplitude before Ba²⁺ application (I_pre) (see A) plotted against duration of closed state Ba²⁺ exposure (n = 4). The dashed line is aligned to the average peak current amplitude upon opening after the 1-s closed state Ba²⁺ exposure and does not have any analytical meaning.