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. 2007 Nov;130(5):479–496. doi: 10.1085/jgp.200709844

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — pH-dependent modulation of single-channel behavior of KcsA under steady-state conditions. (A) Representative traces of single-channel currents recorded within the same patch under different pH conditions. Boxed areas denoting burst activity are shown below at higher resolution. Currents were sampled at 40 kHz and low-pass filtered at 5 kHz. (B) The NPo normalized to the value at pH 3.0 (for three patches) plotted against pH and fitted with the Hill equation yields a pKa of 4.37 ± 0.03 and n_H = 1.51 ± 0.06.