Figure 9.

Global fitting of macroscopic current responses using maximum likelihood-based method to estimate the rate constant in the activation pathway. The rising phase of the macroscopic activation recorded at pH 3.0, 3.5, and 4.0 was simultaneously fit to the scheme (right) to get a direct estimate for the on and off rate for proton binding and the channel opening and closing rate constants. The scheme was constrained to assume that proton binding to all the subunits is equal and independent.