Figure 3.

In vitro aminating (NADH) and deaminating (NAD) GDH activities in wild-type and Vvgdh-NAD;A1 tobacco transgenic lines S2 and S5 and NS and in Slgdh-NAD;B2 tobacco transgenic lines S49 and S77. A and B, Enzyme activities were determined in semipurified plant extracts. C and D, Enzyme activities were determined in the same plant extracts following dialysis. Statistical analysis of data was performed by one-way ANOVA. Asterisks indicate that means (n = 4) of tobacco transgenics are significantly different from the means of the corresponding wild-type and NS for either roots or shoots at P < 0.05 (*) and P < 0.01 (**).