Skip to main content
. 2007 Dec;145(4):1619–1628. doi: 10.1104/pp.107.109876

Figure 4.

Figure 4.

Complementation of the M. truncatula dmi3-1 mutant (TRV25) by OsDMI3. The dmi3-1 mutant of M. truncatula was transformed with OsDMI3 using A. rhizogenes-mediated hairy root transformation (Boisson-Dernier et al., 2001). A full-length cDNA clone of OsDMI3 (AK070533) was cloned into a binary vector modified from pHellsgate8 driven by the CaMV-35S promoter (Helliwell et al., 2002). The binary vector was introduced into the A. rhizogenes strain, ARqua1, and transformed into the roots of the dmi3-1 mutant. A, Transformation of dmi3-1 roots with 35S-OsDMI3 leads to complementation of the nodulation phenotype. B, Defective phenotype of the M. truncatula dmi3-1 mutant inoculated with G. intraradices. The fungal hyphae formed appressoria on the root surface but failed to penetrate the root. eh, Extraradical hypha; ar, arbuscule; ap, appressorium. C, Transformation of dmi3-1 roots with 35S-OsDMI3 leads to complementation of the AM phenotype. D, Expression of MtPT4 in the transgenic dmi3-1 roots inoculated with G. intraradices. The M. truncatula Actin gene was used as a control.