Figure 5.

PIΔC is not defective in ternary complex interaction with AP3 and SEP1. Yeast three-hybrid interactions of AP3, PI, and SEP1. A to C, PI (MIKC) is full-length PI, PI (IKC) is a MADS deleted version of PI, PIΔC is PI lacking the C domain, AP3 (IKC) is AP3 lacking the M domain, and SEP1 (MIKC) is full-length SEP1. PI variants were fused to the GAL4 DNA-binding domain. Full-length SEP1 was fused to the GAL4 activation domain. An unfused MADS deleted version of AP3 was cloned into the pU vector. A, Schematic summarizing yeast genotypes streaked on the plates in B and C. B, Two microliters of yeast culture was plated on Sc-Ura-Leu-Trp-His + Xgal plates. C, Two microliters of yeast culture was plated on Sc-Ura-Leu-Trp plates.