Table 2.
Enzyme kinetics of sPLA2
Km, mM | kcat, sec−1 | |
---|---|---|
Chemically synthesized sPLA2 | 0.96 ± 0.15 | 3.5 ± 0.3 |
Recombinant sPLA2 | 0.94 ± 0.19 | 3.1 ± 0.2 |
Dithio-PC hydrolysis was used to study the kinetic parameters of sPLA2. The data represent the average ± SD of three experiments.