Figure 6.

Influence of actin manipulations on Glc signaling in transient expression assays using Arabidopsis leaf protoplasts. A, Influence of 2 μm LatB on repression of pea RBCS-LUC or Arabidopsis ASN1-Luc without effector, +HXK1, and +HXK1 with 2 mm Glc. Error bars are sd values for at least three replications. Comparable results were obtained also using 10 μm CytoD (data not shown). B, Influence of 2 μm LatB or 10 μm CytoD on F-actin structure in protoplasts of GFP-hTalin seedlings. [See online article for color version of this figure.]