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Immunogold detection of vicilin and TIPs in 3-d-old root tips of pea seedlings. A, Vicilin-positive immunogold labeling (10-nm gold particles) of a vacuole from the inner cortex; cryosections. B, Double immunogold labeling with α-TIP (15-nm gold, arrowheads; α-TIP peptide antiserum [Jauh et al., 1998]) and γ-TIP (5-nm gold, arrows; γ-TIP peptide antiserum [Jauh et al., 1998]) of a PSV from the same cell type. Bars = 150 nm (A); 200 nm (B).
