Figure 5.

DSO 5′ upstream region directed expression. A, GFP expression driven by the DSO 5′ upstream region in the embryo. Expression in radical tip is indicated by an arrow. B to I, GUS expression driven by the DSO 5′ upstream region detected (after 24-h incubation) in: 3-d-old seedling, expression in root cap is indicated by an arrow (B), a higher magnification image of a stained root cap (C); in the vasculature (v) of developing root and at the lateral root primordia (lrp; D); in the emerging lateral root (E); in a 7-d-old seedling (arrow marks lateral root emergence sites; F); in a 15-d-old seedling, expression in the lateral root (lr), main vein (mv), and basal segment (bs) of the leaf is indicated by arrows (G); in the inflorescence (H); and in the developing siliques (I). J, Confocal microscopy images of GFP expression driven by the DSO 5′ upstream region in the adaxial leaf epidermis (i, autofluorescence; ii, GFP signal; iii, merge of GFP with transmission; iv, merge between autofluorescence and GFP). The GFP signal is indicated by arrow. The blue signal marks autofluorescence in the cuticular ledges. K, Confocal microscopy images of GFP expression driven by the DSO 5′ upstream region in the adaxial leaf epidermis showing GFP signal in the trichome base (indicated by arrow) and support cells. L, Images of GFP expression driven by the DSO 5′ upstream region in a free-hand stem cross section. Arrows indicate GFP signal in epidermis. GFP signal was also detected in other stem tissues. Bar = 50 μm in A.