Table I.

Efficiency of RMCE after transformation of Arabidopsis target plant 3164 with Cre and exchange T-DNA constructs

Experiment	Target Plant bar Locusa	Cre Constructb	Exchange T-DNA Construct	No. of Root Explantsc	No. of Kanamycin- Resistant Calli	RMCE Eventsd	RMCE per No. of Kanamycin-Resistant Callie
1	BB	nos-cre	3327	1,557	6	3	0.50
2	BB	nos-cre	3327	1,363	4	1	0.25
3	BB	nos-cre	3327	847	5	3	0.60
		bm-cre	3327	860	3	2	0.67
4	B−	nos-cre	3327	664	5	2	0.40
		bm-cre	3327	755	1	1	1.00
5	B−	nos-cre	3327	1,725	8	4	0.50
		nos-cre	3732	1,705	8	3	0.38
6	B−	nos-cre	3327	1,080	6	2	0.33
		bm-cre	3327	977	2	0	0
		nos-cre	3732	1,030	2	1	0.50
		bm-cre	3732	1,021	0	0	0

^aHemizygous (B−) or homozygous (BB) plant material.

^bbm, BigMac promoter.

^cRoots were cut into explants of 2 to 3 mm in size; up to 500 explants were plated per petri dish.

^dRMCE events determined by PCR using primers p1 and r4.

^eRMCE events per no. of kanamycin-resistant calli.