Figure 7.

Expression of various proteins from the TRBO vector. N. benthamiana leaves were agroinoculated with pJL-TRBO vectors expressing various genes. Total soluble protein extracts were prepared approximately 5 dpi. Equal volumes of extract were loaded per lane. In some cases, proteins were expressed as fusions to a peptide tag of His-6-hemaglutinin peptide (duplicated), H₆HA₂. A, Coomassie Blue-stained SDS-PAGE gel of extracts. B, Western-blot (immunoblot) analysis of extracts using anti-HA peptide primary antibody. Lanes: M, molecular mass marker; M2, SeeBlue (Invitrogen) molecular mass marker; H, healthy plant extract. Extracts from tissue infected with JL-TRBO vector expressing the following genes: 1, Phytopthora infestans Avr3a (15 kD); 2, Aequorea victoria GFP (27 kD); 3, GFP:H₆HA₂ fusion (31 kD); 4, Arabidopsis adenosine kinase (38 kD); 5, 10th type III (FN10) domain from human fibronectin:H₆HA₂ (13 kD); 6, tomato RCR-3 proteinase:H₆HA₂ (42 kD); 7, tomato P69b proteinase:H₆HA₂ (73 kD). White and gray circles denote location of FN10 and RCR-3 proteins on Coomassie Blue-stained gel, respectively. [See online article for color version of this figure.]