Figure 3.
Determining the CRE efficiency in module 1 (common germline or double germline CRE functionality). Yellow triangle, lox site; C, codA cassette, counter-selection on 5FC; H, hpt cassette, resistance to hygromycin; N, nptII cassette, resistance to kanamycin; between the lox sites, a 1.9-kb promoter fragment of the Arabidopsis AP1 gene is present upstream of the cre gene containing an intron. HR/S, Ratio resistant-to-sensitive T2 plants on hygromycin (n = ±200 seeds). X, Efficiency with which the CRE/lox recombination reaction occurs in the common germline (indicated as a red bar) leading to a marker-free gamete. A complete overview of the segregation data are given in Supplemental Table S3. For each line, X was calculated from the observed HR/S ratio.