Histochemical GUS staining of 2-week-old seedlings. Transformants with low GUS activity (PA22, PA25, PA29, and TCA29-5) had a patchy pattern, while transformants with a stable and high GUS expression (TCA22-7, TCA25-9, HCA29, and TCA29-4) were uniformly stained. Nontransgenic Col-0 plants had no blue staining (De Buck et al., 2004).