Table I.
Transformant | T-DNA | No. of T-DNAsa | Configurationsb |
---|---|---|---|
PA23 | Ksb | 2 | Read through |
PA22 | Ksb | 4 | IRRB, read through |
PA25 | Ksb | 2 | IRRB |
PA29 | Ksb | 4–6 | At least one IRRB |
PA1 | Ksb | 4–6 | At least one IRRB, read through |
PA5 | Ksb | 4–6 | IRRB, read through |
PA16 | Ksb | 3–4 | Read through |
CRE13 | Cre | ND | ND |
HSC6 | Cre | ND | ND |
T-DNA copy number determined on the basis of the number of T-DNA/plant DNA junction fragments on the DNA gel blots.
Read through, vector backbone sequence inserted between two T-DNAs in tandem orientation, presumably because of read through at the LB.