Figure 2.

Selected phenotypes observed with silencing of tomato ESTs. A, Silencing of SlRibosomal protein L11 like, Slα-tubulin, SlHSP70, and SlPolyubiquitin causes death. B, Silencing SlMAP3K epsilon disrupts leaf development resulting in severe crinkling. C, Reduction in SlGeranylgeranyl reductase levels causes yellowing of leaves. D, Reduced SlPlastidic aldolase causes photobleaching and a variegated appearance. E, Decreased SlCyclin-dependent protein kinase, p34cdc2, levels cause severe stunting. F, Homeotic transformations of floral tissue in SlTAG1-silenced plants. G, mRNA levels in control (left) versus silenced (right) tissue indicate effective silencing of genes indicated. Number of PCR cycles is indicated above lanes. Lane M is the DNA size marker. C is no RT control. Actin is a loading control.