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. 2007 Dec;145(4):1100–1109. doi: 10.1104/pp.107.106641

Figure 1.

Figure 1.

Schematic representations of pSITE vectors. A, All modified pSAT6 cassettes were cloned into the pRCS2-ocs-nptII binary vector at the PI-Psp1 site. The ability to select transgenic plant cells is conferred by the nptII gene, the expression of which is controlled by the ocs promoter (P-OCS) and terminator (T-OCS). B, C-series pSITE vectors for Gateway recombination-mediated construction of binary vectors for expression of proteins of interest fused to the carboxy termini of AFPs. C, N-series pSITE vectors for Gateway recombination-mediated construction of binary vectors for expression of proteins of interest fused to the amino termini of AFPs. D, 0-series pSITE vectors for Gateway recombination-mediated construction of binary vectors for expression of native proteins. Protein expression is controlled by a duplicated CaMV 35S promoter (2X35S) and a tobacco etch virus translational leader (TL). All vectors employ the CaMV35S transcriptional terminator (TER). Nco1*, This restriction site was deleted to create the pSITE-NB and pSITE-0B vectors, thereby allowing translation to initiate at the native start codon on the gene of interest. This figure is a revision of that appearing in Chakrabarty et al. (2007). [See online article for color version of this figure.]