Physical maps of R. marinus dnaB gene. On the top line, the dnaB gene is transcribed from left to right, while the neighboring β-mannanase gene (stippled box) is transcribed from right to left. The dnaB gene consists of extein coding sequences (hatched boxes) and intein coding sequence (solid box). In the middle, a solid line marks DNA sequence determined in this study, a dashed line marks DNA sequence determined previously by others, and a short thick line (P) marks a 600-bp DNA probe that was used in cloning the dnaB gene. The lower line shows restriction sites used in this study.