Figure 3. Level of viral production and calculated levels of viral inhibition measured by the autologous T cell co-culture method in the absence of NL4-3 superinfection.

PBMC were isolated from blood from HIV+ donors and stimulated with bi-specific antibodies to produce pure CD4⁺ and CD8⁺ T lymphocyte cultures. After 14 days the CD8⁺ T lymphocytes were added to the wells containing autologous CD4⁺ cells at three different ratios (0.5:1, 1:1, and 2:1), each in triplicate. Media only was added to three wells as a positive control for maximum viral production in the absence of CD8⁺ T lymphocytes. Viral production was measured by p24 ELISA at three time points, as described in Materials and Methods. Panel a shows the actual p24 production at three different time-points from HIV+ Donor 1 and Panel b shows the calculated percent inhibition using the formula described in the Figure 2 legend.