Fig. 3.

The amount of protein greatly influences the delivery efficiency. Cell sorting experiments were carried out onto HeLa cells after intracellular protein delivery. A volume of 4 μL of delivery reagent was added to various amounts of R-PE (1–8 μg) or 1 μg of BSA (negative control, Ctrl). Complexes were added to cells and further incubated for 20 h before FACS analysis. Gates were set to distinguish between autofluorescence of control cells and highly fluorescent cells. Means of percentage of fluorescent cells ± SEM are shown (a), n = 3 per point. Results are also presented as the percentage of gated fluorescent cells per mean intensity of fluorescence (b), n = 3 per point