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The incubation time of protein/delivery reagent complexes with cells influences the delivery efficiency. Delivery reagent (4 μL) was added to R-PE (1 μg) or BSA (1 μg, Ctrl) to form complexes. Complexes were thereafter added onto HeLa cells and further incubated for the indicated time (30 min to 6 h). FACS analysis was carried out 16 h post-delivery. The experiment was carried out as described in the figure 3
