Fig. 5.

Kinetic of intracellular R-PE delivery into human primary fibroblasts. One μg of R-PE diluted in 100 μL Hepes buffer (20 mM, pH 7.4) was incubated with 4 μL of delivery reagent for 15 min at room temperature. The cells were analyzed 4 (b), 8 (c) and 20 h (d) after addition of protein/reagent complexes onto cells. As a control (a), R-PE alone was added onto human primary fibroblasts and analysed 20 h after addition of the protein onto cells