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pH–kcat/KM profile for the cleavage of 6-FAM–dArUdGdA–6-TAMRA by ONC. Assays were performed at 23 °C in 1.0 mM buffer containing NaCl (1.0 M). Determination of kcat/KM values was performed in triplicate. Data were fitted to equation (1) by non-linear least squares regression analysis to give pKa values of 5.84 ± 0.05 and 6.77 ± 0.04.
