Skip to main content
. Author manuscript; available in PMC: 2009 Jan 4.
Published in final edited form as: J Mol Biol. 2007 Oct 4;375(1):165–177. doi: 10.1016/j.jmb.2007.09.089

Figure 4.

Figure 4

Effect of Thr89 and Glu91 on the substrate specificity of ONC. Bars indicate the effect of replacing Thr89 or Glu91 on the value of kcat/KM for the cleavage of 6-FAM–dArUdGdA–6-TAMRA (UpG) and 6-FAM–dArUdAdA–6-TAMRA (UpA). The guanine:adenine preference of T89R ONC is 3.3 × 103-fold greater than that of T89N/E91A ONC (Table 2).