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. Author manuscript; available in PMC: 2008 Oct 26.
Published in final edited form as: Immunity. 2007 Oct 11;27(4):610–624. doi: 10.1016/j.immuni.2007.08.015

Figure 1. Disruption of E-cadherin-mediated clusters results in DC maturation.

Figure 1

(Panel A) DCs matured after cluster disruption (CD) exhibited similar mophological changes as induced by LPS. DCs matured by CD or LPS were labeled for MHC II (red) and the lysosomal marker Lamp 2 (green). (Panel B) Anti-E-cadherin antibodies can block DC maturation induced by CD. BMDCs were prepared as described and CD11c+ DCs were purified at day 6 and replated at 5 × 105 cells/ml. Treatment with an anti-E-cadherin mAb (Sigma) but not isotype-matched anti-CD11b mAb or mouse IgG inhibited the upregulation of CD86.