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. Author manuscript; available in PMC: 2008 Oct 26.
Published in final edited form as: Immunity. 2007 Oct 11;27(4):610–624. doi: 10.1016/j.immuni.2007.08.015

Figure 5. CD-matured murine BMDCs upregulated CCR7 without inflammatory cytokine induction.

Figure 5

(Panel A) CD-matured murine BMDCs did not induce inflammatory cytokines IL-1β, IL-6, IL-12p40 and TNFα. Real-time RT-PCRs were performed on total RNA isolated from DCs treated with either LPS or CD for the indicated times, the expression of each gene then was normalized to β-actin expression. (Panel B) CD-matured BMDCs express elevated level of surface CCR7. DCs untreated or matured by either CD or LPS were subjected to FACS analysis. (Panel C) Addition of LPS after cluster disruption synergistically enhances or inhibits cytokine production. Real-time RT-PCRs were performed and analyzed as described in Panel A. Cluster-disrupted DCs were stimulated with LPS simultaneously (CD+LPS) or LPS was added 14-18 hr afterwards for the indicated times (CD--->LPS). Results from one of three different sets of samples are shown.