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. 1997 Jul 22;94(15):7862–7867. doi: 10.1073/pnas.94.15.7862

Figure 2.

Figure 2

Localization of mE2 protein in the cytoplasm and nucleus of mammalian cells. Histochemistry showing endogenous mE2 protein and transiently expressed GFP-mE2 fusion protein localizes in both the cytoplasm and nucleus of human cells. (A) Detection of green fluorescence in both the cytoplasm and nucleus of 293T cells expressing GFP-mE2 fusion protein. (B) mE2-like immunoreactivity was detected in red fluorescence. Two cells expressing GFP-mE2 fusion protein and other cells having endogenous-E2 show the same distribution pattern, both in the cytoplasm and nucleus. (C) The same cells used in A and B were stained with 4′,6-diamidino-2-phenylindole to visualize the nucleus. (D) mE2-like immunoreactivity was detected in red fluorescence in the cytoplasm and nucleus of HeLa cells.