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. 1997 Jul 22;94(15):7868–7872. doi: 10.1073/pnas.94.15.7868

Figure 2.

Figure 2

A28-RGS14 gene encodes a novel conserved RGS protein. (A) Predicted protein sequences of human and mouse A28-RGS14p protein. (B) Alignment of A28-RGS14p amino acid sequence and that of related mammalian, yeast, and C. elegans RGS protein family members. Shaded areas indicate a 130-amino acid core domain with highest sequence conservation and a predicted α-helical structure. (C) Tissue expression pattern of the A28-RGS14 gene. A human multitissue Northern blot (CLONTECH) was hybridized with a 32P-labeled A28-RGS14 cDNA probe as described in Fig. 1A. (D) In vitro interaction of A28-RGS14p with Gαi2, but not Gαs subunits of heterotrimeric G proteins. The in vitro-translated 35S-labeled Gα proteins were coimmunoprecipitated with purified His-tagged A28-RGS14p (His-A28) and anti-A28-RGS14p antibody 8D12 (Ab-8D12; Y.C., L.B., and N.K., unpublished results). Lanes: 1, in vitro-translated Gα; 2, coimmunoprecipitation of Gα with His-A28-RGS14p using Ab-8D12; 3, coimmunoprecipitation of Gα without His-A28-RGS14p; 4, coimmunoprecipitation of in vitro-translated Gα with purified His-tagged p53 (His-p53) and its specific antibody (Ab-1, Oncogene Science); 5, coimmunoprecipitation of in vitro-translated hdm2 protein with His-A28-RGS14p and Ab-8D12. m.w.m., Molecular mass markers.