Figure 3.

S. cerevisiae and human Dol-P-Man synthase genes allow growth of haploid S. pombe dpm1⁺::his7⁺ cells. Heterozygous dpm1⁺::his7⁺/dpm1⁺ diploids harboring pScDPM1, pHsDPM1, pSpdpm1⁺, or the vector pREP2 were allowed to sporulate, and the meiotic progeny were tested for growth on medium selective for histidine and uracil prototrophy. Segregants from the dpm1⁺::his7⁺/dpm1⁺ diploid harboring pREP2 were also plated on medium containing histidine to select for wild-type haploids containing pREP2.