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. 1997 Jul 22;94(15):7879–7884. doi: 10.1073/pnas.94.15.7879

Figure 1.

Figure 1

Role of SRC-1A in PR transactivation in vitro. Effects of purified GST control, GST-SRC-1(1216–1441), and GST-SRC-1(384–842) proteins were analyzed in a PR-driven in vitro transcription assay. Assays were performed in the presence of vehicle (lanes 1, 3, 5, and 9) or 10−7 M progesterone (lanes 2, 4, 6–8, 10, and 11) and various concentrations of GST fusion proteins (presented in pmol). Upper arrow indicates correctly initiated transcripts from the (PRE)2TATA-G-free 290-bp reporter. The AdML arrow indicates correctly initiated transcripts from the AdML-G-free 200-bp internal control. Gels were quantified using a Betagen blot scanner.