Figure 4.

Direct interaction of α-TQH₂ with α-TO^⋅ in LDL. α-TO^⋅ was generated in LDL (7.2 μM in apoB) by incubation with SLO (1 mg/ml) at 37°C, and recorded by EPR spectroscopy before (A) and after (B) addition of 10 μM of α-TQH₂ at the same region of field as detailed in the text. (C) The changes in UV_{234 nm} absorbance associated with the conversion of linoleic acid (0.1 mM in PBS) to linoleic acid hydroperoxide by SLO (1 mg/ml) in the absence (dashed line) and presence (solid line) of 10 μM α-TQH₂. The data shown are from a single experiment, representative of three separate experiments.