Figure 2.

Cytosolic and mitochondrial GlnRS are distinct. (A) Inferred secondary strucures of cytosol-specific (Cyt) and mitochondrial (Cyt/Mit) tRNAs^Gln of L. tarentolae. Nucleotides in the mitochondrial tRNA^Gln that are different from the cytosolic ones are boxed. (B) Cytosol-specific tRNA^Gln (CUG) and mitochondrial tRNA^Gln (UUG) of L. tarentolae were charged with ¹⁴C-glutamine using 60 μg each of RNA-depleted cytosolic (Cyt) or mitochondrial matrix (Mit) fractions. As a control, the reactions were performed without adding the tRNAs (no tRNA). Bars indicate mean values (±SD) of independent aminoacylation reactions (n = 4–8) using at least two independently prepared cytosolic or mitochondrial extracts. Y axis as Fig. 1.