Table 1.
Time course of RIP14 activation by TTNPB and tRA
| h
|
|||||
|---|---|---|---|---|---|
| 0 | 1 | 3 | 6 | 24 | |
| RIP14 + RXR | |||||
| + 5 μM TTNPB | 0.2 ± 0.0 | 0.2 ± 0.1 | 1.3 ± 0.5 | 10.4 ± 1.0 | 100.0 ± 0.0 |
| + 5 μM tRA | 0.3 ± 0.0 | 0.3 ± 0.0 | 2.8 ± 2.6 | 3.8 ± 0.5 | 100.0 ± 0.0 |
| Endogenous RAR | |||||
| + 5 μM TTNPB | 0.4 ± 0.2 | 0.4 ± 0.2 | 5.7 ± 2.0 | 21.5 ± 4.8 | 100.0 ± 0.0 |
| + 5 μM tRA | 0.4 ± 0.0 | 0.6 ± 0.1 | 6.3 ± 1.0 | 54.1 ± 12.7 | 100.0 ± 0.0 |
JEG-3 cells were cotransfected as in Fig. 1 with either wild-type RIP14 plus RXR and hsp27 EcREx5 ΔMTV–luciferase or CDM 8 and β2RARE x3 TK luc reporter; 24 h after transfection, the cells were treated with either 5 μM TTNPB or 5 μM tRA and harvested at the indicated time point. The luciferase activity at the indicated time points is presented by comparison to that observed at 24 h and is the average of three separate experiments. For RIP14 plus RXR, the relative activation at 24 h was 530- ± 110-fold with TTNPB and 340- ± 46-fold with tRA. For the endogenous RARs, the relative activation at 24 h was 160- ± 45-fold with TTNPB and 270- ± 34-fold with tRA.