Figure 5.
Effect of α subunit gene inactivations on receptor-mediated IK+Ach activation. (A) Comparison of IK+Ach activity expressed as NPo before (basal) and after addition of carbachol (10 μM) in WT, α-null cells, and αi-R cells in which the targeted gene has been reexpressed. Bars are means ± SE for 10–20 recordings for WT and αi-null cells and 4 to 5 recordings for αi-R, αo-null, and αq-null cells. (B) Western blot of membrane protein from undifferentiated WT, αi-null cells, and αi-R cells in which the targeted gene (αi2 or αi3) has been reexpressed demonstrate the absence of protein in the null cells but significant protein in the rescued (αi-R) cell lines.