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Fluorescence decay of single TMR molecules in the 1:1 tRNAPhe–TMR adduct (Upper) and in the polymethylmethacrylate film (Lower). The nonexponential decay kinetics of the tRNAPhe–TMR adduct is evident when the signal is compared with the single exponential decay of the fluorescence of rhodamine in a polymer film. PMMA, polymethylmethacrylate.
