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. 1997 Jul 22;94(15):7948–7953. doi: 10.1073/pnas.94.15.7948

Figure 4.

Figure 4

Requirement of the putative NLS for nuclear import. (A) Basonuclin sequence with a FLAG tag and sites of three different deletions, beginning at indicated residue numbers and extending to C-terminal end (residue 994). (B) FLAG-basonuclins each bearing one of the three deletions were expressed for 22 hr after transfection in the SSC-13 line of human keratinocytes (7). The expressed basonuclins were visualized by immunostaining with mAb against the FLAG tag. Length of deletion sequences is indicated in parentheses. Two deletion proteins lacking the NLS, Δ(445–994) and Δ(202–994), were localized in the cytoplasm at a higher concentration than in the nucleus. (C) Examples of nuclear and cytoplasmic basonuclin from experiment described in B. Cell number scored for each transfection was: WT, 82; Δ(708–994), 61; Δ(445–994), 57; and Δ(202–994), 30.