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Phosphorylation in vitro of a basonuclin fragment containing serine substitutions. Five micrograms of the polypeptide used in Fig. 5, each bearing a substitution for one or two serines, was incubated with 17 μg of keratinocyte extract in the reaction mixture for 15 min at 25°C. Radioactivity was detected by autoradiography and protein by Coomassie blue staining. The three serine residues are numbered. (Upper) Aspartic acid substitutions. (Lower) Alanine substitutions.
